Correlative Microscopy using Serial Blockface Scanning EM
نویسندگان
چکیده
منابع مشابه
D correlative light and electron microscopy of cultured cells using serial blockface scanning electron microscopy Matthew
The processes of life take place in multiple dimensions, but imaging these processes in even three dimensions is challenging. Here we describe a workflow for 3D correlative light and electron microscopy (CLEM) of cell monolayers using fluorescence microscopy to identify and follow biological events, combined with serial blockface scanning electron microscopy to analyse the underlying ultrastruc...
متن کامل3D correlative light and electron microscopy of cultured cells using serial blockface scanning electron microscopy
The processes of life take place in multiple dimensions, but imaging these processes in even three dimensions is challenging. Here, we describe a workflow for 3D correlative light and electron microscopy (CLEM) of cell monolayers using fluorescence microscopy to identify and follow biological events, combined with serial blockface scanning electron microscopy to analyse the underlying ultrastru...
متن کاملTOOLS AND TECHNIQUES 3D correlative light and electron microscopy of cultured cells using serial blockface scanning electron microscopy
The processes of life take place in multiple dimensions, but imaging these processes in even three dimensions is challenging. Here, we describe a workflow for 3D correlative light and electron microscopy (CLEM) of cell monolayers using fluorescence microscopy to identify and follow biological events, combined with serial blockface scanning electron microscopy to analyse the underlying ultrastru...
متن کاملTOOLS AND TECHNIQUES SPECIAL ISSUE: 3D CELL BIOLOGY 3D correlative light and electron microscopy of cultured cells using serial blockface scanning electron microscopy
The processes of life take place in multiple dimensions, but imaging these processes in even three dimensions is challenging. Here, we describe a workflow for 3D correlative light and electron microscopy (CLEM) of cell monolayers using fluorescence microscopy to identify and follow biological events, combined with serial blockface scanning electron microscopy to analyse the underlying ultrastru...
متن کاملCorrelative Photoactivated Localization and Scanning Electron Microscopy
The ability to localize proteins precisely within subcellular space is crucial to understanding the functioning of biological systems. Recently, we described a protocol that correlates a precise map of fluorescent fusion proteins localized using three-dimensional super-resolution optical microscopy with the fine ultrastructural context of three-dimensional electron micrographs. While it achieve...
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ژورنال
عنوان ژورنال: Microscopy and Microanalysis
سال: 2015
ISSN: 1431-9276,1435-8115
DOI: 10.1017/s1431927615007692